Pilot study on the value of Raman spectroscopy in the entity assignment of salivary gland tumors
Zitieren Sie bitte immer diese URN: urn:nbn:de:bvb:20-opus-264736
- Background The entity assignment of salivary gland tumors (SGT) based on histomorphology can be challenging. Raman spectroscopy has been applied to analyze differences in the molecular composition of tissues. The aim of this study was to evaluate the suitability of RS for entity assignment in SGT. Methods Raman data were collected in deparaffinized sections of pleomorphic adenomas (PA) and adenoid cystic carcinomas (ACC). Multivariate data and chemometric analysis were completed using the Unscrambler software. Results The Raman spectraBackground The entity assignment of salivary gland tumors (SGT) based on histomorphology can be challenging. Raman spectroscopy has been applied to analyze differences in the molecular composition of tissues. The aim of this study was to evaluate the suitability of RS for entity assignment in SGT. Methods Raman data were collected in deparaffinized sections of pleomorphic adenomas (PA) and adenoid cystic carcinomas (ACC). Multivariate data and chemometric analysis were completed using the Unscrambler software. Results The Raman spectra detected in ACC samples were mostly assigned to nucleic acids, lipids, and amides. In a principal component-based linear discriminant analysis (LDA) 18 of 20 tumor samples were classified correctly. Conclusion In this proof of concept study, we show that a reliable SGT diagnosis based on LDA algorithm appears possible, despite variations in the entity-specific mean spectra. However, a standardized workflow for tissue sample preparation, measurement setup, and chemometric algorithms is essential to get reliable results.…
Autor(en): | Till Jasper MeyerORCiD, Elena Gerhard-Hartmann, Nina Lodes, Agmal Scherzad, Rudolf Hagen, Maria Steinke, Stephan Hackenberg |
---|---|
URN: | urn:nbn:de:bvb:20-opus-264736 |
Dokumentart: | Artikel / Aufsatz in einer Zeitschrift |
Institute der Universität: | Medizinische Fakultät / Klinik und Poliklinik für Hals-, Nasen- und Ohrenkrankheiten, plastische und ästhetische Operationen |
Medizinische Fakultät / Pathologisches Institut | |
Medizinische Fakultät / Lehrstuhl für Tissue Engineering und Regenerative Medizin | |
Sprache der Veröffentlichung: | Englisch |
Titel des übergeordneten Werkes / der Zeitschrift (Englisch): | PLoS One |
Erscheinungsjahr: | 2021 |
Band / Jahrgang: | 16 |
Heft / Ausgabe: | 9 |
Aufsatznummer: | e0257470 |
Originalveröffentlichung / Quelle: | PLoS One (2021) 16:9, e0257470. https://doi.org/10.1371/journal.pone.0257470 |
DOI: | https://doi.org/10.1371/journal.pone.0257470 |
Allgemeine fachliche Zuordnung (DDC-Klassifikation): | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Freie Schlagwort(e): | Head and neck cancers; cancers and neoplasms; lipids; malignant tumors; raman spectroscopy; salivary gland tumors; salivary glands; surgical oncology |
Datum der Freischaltung: | 05.05.2022 |
Sammlungen: | Open-Access-Publikationsfonds / Förderzeitraum 2021 |
Lizenz (Deutsch): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |