cAMP signaling microdomains and their observation by optical methods
Please always quote using this URN: urn:nbn:de:bvb:20-opus-118252
- The second messenger cyclic AMP (cAMP) is a major intracellular mediator of many hormones and neurotransmitters and regulates a myriad of cell functions, including synaptic plasticity in neurons. Whereas cAMP can freely diffuse in the cytosol, a growing body of evidence suggests the formation of cAMP gradients and microdomains near the sites of cAMP production, where cAMP signals remain apparently confined. The mechanisms responsible for the formation of such microdomains are subject of intensive investigation. The development of opticalThe second messenger cyclic AMP (cAMP) is a major intracellular mediator of many hormones and neurotransmitters and regulates a myriad of cell functions, including synaptic plasticity in neurons. Whereas cAMP can freely diffuse in the cytosol, a growing body of evidence suggests the formation of cAMP gradients and microdomains near the sites of cAMP production, where cAMP signals remain apparently confined. The mechanisms responsible for the formation of such microdomains are subject of intensive investigation. The development of optical methods based on fluorescence resonance energy transfer (FRET), which allow a direct observation of cAMP signaling with high temporal and spatial resolution, is playing a fundamental role in elucidating the nature of such microdomains. Here, we will review the optical methods used for monitoring cAMP and protein kinase A (PKA) signaling in living cells, providing some examples of their application in neurons, and will discuss the major hypotheses on the formation of cAMP/PKA microdomains.…
Author: | Davide Calebiro, Isabella Maiellaro |
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URN: | urn:nbn:de:bvb:20-opus-118252 |
Document Type: | Journal article |
Faculties: | Medizinische Fakultät / Institut für Pharmakologie und Toxikologie |
Fakultät für Biologie / Rudolf-Virchow-Zentrum | |
Language: | English |
Parent Title (English): | Frontiers in Cellular Neuroscience |
ISSN: | 1662-5102 |
Year of Completion: | 2014 |
Volume: | 8 |
Pagenumber: | 350 |
Source: | Frontiers in Cellular Neuroscience 8:350. doi: 10.3389/fncel.2014.00350 |
DOI: | https://doi.org/10.3389/fncel.2014.00350 |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 571 Physiologie und verwandte Themen |
6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 611 Menschliche Anatomie, Zytologie, Histologie | |
Tag: | G protein-coupled receptor; cyclic AMP; fluorescence resonance energy transfer; neurons; signaling microdomain |
Release Date: | 2015/10/02 |
Licence (German): | CC BY: Creative-Commons-Lizenz: Namensnennung |