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CD56 Is a Pathogen Recognition Receptor on Human Natural Killer Cells

Please always quote using this URN: urn:nbn:de:bvb:20-opus-170637
  • Aspergillus (A.) fumigatus is an opportunistic fungal mold inducing invasive aspergillosis (IA) in immunocompromised patients. Although antifungal activity of human natural killer (NK) cells was shown in previous studies, the underlying cellular mechanisms and pathogen recognition receptors (PRRs) are still unknown. Using flow cytometry we were able to show that the fluorescence positivity of the surface receptor CD56 significantly decreased upon fungal contact. To visualize the interaction site of NK cells and A. fumigatus we used SEM, CLSMAspergillus (A.) fumigatus is an opportunistic fungal mold inducing invasive aspergillosis (IA) in immunocompromised patients. Although antifungal activity of human natural killer (NK) cells was shown in previous studies, the underlying cellular mechanisms and pathogen recognition receptors (PRRs) are still unknown. Using flow cytometry we were able to show that the fluorescence positivity of the surface receptor CD56 significantly decreased upon fungal contact. To visualize the interaction site of NK cells and A. fumigatus we used SEM, CLSM and dSTORM techniques, which clearly demonstrated that NK cells directly interact with A. fumigatus via CD56 and that CD56 is re-organized and accumulated at this interaction site time-dependently. The inhibition of the cytoskeleton showed that the receptor re-organization was an active process dependent on actin re-arrangements. Furthermore, we could show that CD56 plays a role in the fungus mediated NK cell activation, since blocking of CD56 surface receptor reduced fungal mediated NK cell activation and reduced cytokine secretion. These results confirmed the direct interaction of NK cells and A. fumigatus, leading to the conclusion that CD56 is a pathogen recognition receptor. These findings give new insights into the functional role of CD56 in the pathogen recognition during the innate immune response.show moreshow less

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Metadaten
Author: Sabrina Ziegler, Esther Weiss, Anna-Lena Schmitt, Jan SchlegelORCiD, Anne Burgert, Ulrich TerpitzORCiD, Markus Sauer, Lorenzo Moretta, Simona SivoriORCiD, Ines Leonhardt, Oliver Kurzai, Hermann Einsele, Juergen Loeffler
URN:urn:nbn:de:bvb:20-opus-170637
Document Type:Journal article
Faculties:Medizinische Fakultät / Medizinische Klinik und Poliklinik II
Fakultät für Biologie / Theodor-Boveri-Institut für Biowissenschaften
Language:English
Parent Title (English):Scientific Reports
Year of Completion:2017
Volume:7
Issue:6138
Source:Scientific Reports 2017, 7:6138. DOI: 10.1038/s41598-017-06238-4
DOI:https://doi.org/10.1038/s41598-017-06238-4
Pubmed Id:http://www.ncbi.nlm.nih.gov/pubmed?term=28733594
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Tag:Aspergillus fumigatus; fungal infection; natural killer cells; pattern recognition receptors
Release Date:2019/10/02
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International