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Long-term fate of etoposide-induced micronuclei and micronucleated cells in Hela-H2B-GFP cells
Please always quote using this URN: urn:nbn:de:bvb:20-opus-235039
- Micronuclei are small nuclear cellular structures containing whole chromosomes or chromosomal fragments. While there is a lot of information available about the origin and formation of micronuclei, less is known about the fate of micronuclei and micronucleated cells. Possible fates include extrusion, degradation, reincorporation and persistence. Live cell imaging was performed to quantitatively analyse the fates of micronuclei and micronucleated cells occurring in vitro. Imaging was conducted for up to 96 h in HeLa-H2B-GFP cells treated withMicronuclei are small nuclear cellular structures containing whole chromosomes or chromosomal fragments. While there is a lot of information available about the origin and formation of micronuclei, less is known about the fate of micronuclei and micronucleated cells. Possible fates include extrusion, degradation, reincorporation and persistence. Live cell imaging was performed to quantitatively analyse the fates of micronuclei and micronucleated cells occurring in vitro. Imaging was conducted for up to 96 h in HeLa-H2B-GFP cells treated with 0.5, 1 and 2 µg/ml etoposide. While a minority of micronuclei was reincorporated into the main nucleus during mitosis, the majority of micronuclei persisted without any alterations. Degradation and extrusion were observed rarely or never. The presence of micronuclei affected the proliferation of the daughter cells and also had an influence on cell death rates. Mitotic errors were found to be clearly increased in micronucleus-containing cells. The results show that micronuclei and micronucleated cells can, although delayed in cell cycle, sustain for multiple divisions.…
Author: | Hauke Reimann, Helga Stopper, Henning HintzscheORCiD |
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URN: | urn:nbn:de:bvb:20-opus-235039 |
Document Type: | Journal article |
Faculties: | Medizinische Fakultät / Institut für Pharmakologie und Toxikologie |
Language: | English |
Parent Title (English): | Archives of Toxicology |
ISSN: | 0340-5761 |
Year of Completion: | 2020 |
Volume: | 94 |
Pagenumber: | 3553–3561 |
Source: | Archives of Toxicology (2020) 94:3553–3561. https://doi.org/10.1007/s00204-020-02840-0 |
DOI: | https://doi.org/10.1007/s00204-020-02840-0 |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Tag: | DNA damage; cell fate; etoposide; live imaging; micronuclei |
Release Date: | 2021/06/11 |
Licence (German): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |