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The term "speech chronemics" is introduced to characterize a research strategy which extracts from the physical qualities of the speech signal only the pattern of ons ("speaking") and offs ("pausing"). The research in this field can be structured into the methodological dimension "unit of time", "number of speakers", and "quality of the prosodic measures". It is shown that a researcher's actual decision for one method largely determines the outcome of his study. Then, with the Logoport a new portable measurement device is presented. It enables the researcher to study speaking behavior over long periods of time (up to 24 hours) in the normal environment of his subjects. Two experiments are reported. The first shows the validity of articulation pauses for variations in the physiological state of the organism. The second study proves a new betablocking agent to have sociotropic effects: in a long-term trial socially high-strung subjects showed an improved interaction behavior (compared to placebo and socially easy-going persons) in their everyday life. Finally, the need for a comprehensive theoretical foundation and for standardization of measurement situations and methods is emphasized.
Wenn Strafe und Schadenersatz gemeinsam im Urteil verwendet werden, bilden sie methodisch einen besonderen multi variaten Urteils komplex, weil auch ihre Summe psychologisch interpretierbar ist. Anhand der Effekte dieses Verbunds, den auch das Zivil- und Strafrecht kennt, wurde geprüft, ob der Einsatz eines Doppel-Urteils für die Erforschung sozialer Kognitionen erforderlich ist. Dazu beurteilten Pbn in zwei Untersuchungen Informationen über schädigende Handlungen mit einem univariaten Ersatz-Urteil (N = 124) bzw. mit dem Doppel-Urteil von Ersatz und Strafe (N = 143). Als Folge der Einführung des Doppel-Urteils ergab sich, daß die Häufigkeiten von Über kompensation für absichtliche Schädigungen erheblich reduziert wurden. Jedoch wurden zugleich die Häufigkeiten von vollem Ersatz bei diesen Bedingungen beträchtlich erhöht. Bei versehentlichen Schädigungen hatte die Einführung des Doppel-Urteils keinen Effekt. Weiterhin blieb dort der univariate Befund multipler Modalwerte erhalten. In den Mittelwerten hatte das Mitverschulden des Geschädigten nur im Schadensersatz-Urteil einen Effekt, nicht jedoch in den Strafurteilen. Außerdem blieb die für die Schadensersatz- Urteile charakteristische Non-Additivität von Verschulden und Mitschuld bestehen.
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PtK2 cells in which pore complex-mediated transport is blocked by microinjection early in mitosis of a monoclonal antibody (specific for an Mr 68000 pore complex glycoprotein) or of wheat germ agglutinin (WGA) complete cytokinesis. However, their nuclei remain stably arrested in a telophase-like organization characterized by highly condensed chromatin and the absence of nucleoli, indicating a requirement for pore-mediated transport for the reassembly of interphase nuclei. We have now examined this requirement more closely by monitoring the behavior of individual nuclear macromolecules in microinjected cells using immunofluorescence microscopy and have investigated the effect of microinjecting the antibody or WGA on cellular ultrastructure. The absence of nuclear transport did not affect the sequestration into daughter nuclei of components such as DNA, DNA topoisomerase I and the nucleolar protein fibrillarin that are carried through mitosis on chromosomes. On the other hand, lamins, snRNAs and the p68 pore complex glycoprotein, all cytoplasmic during mitosis, remained largely cytoplasmic in the telophase-arrested cells. Electron microscopy showed the nuclei to be surrounded by a doublelayered membrane with some inserted pore complexes. In addition, however, a variety of membranous structures with associated pore complexes was regularly noted in the cytoplasm, suggesting that chromatin may not be essential for the postmitotic formation of pore complexes. We propose that cellular compartmentalization at telophase is a two-step process. First, a nuclear envelope tightly encloses the condensed chromosomes, excluding non-selectively all macromolecules not associated with the chromosomes. Interphase nuclear organization is then progressively restored by selective pore complex-mediated uptake of nuclear proteins from the cytoplasm.
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Dications 1 derived from 2,2-bipyridine are found to exist as fully reversible two step redox systems with persistent radical ions (SEM) of high thermodynamic stability, if the bridge X forces the two pyridine rings into co planar positions. The well known derivative 1a (Diquat R) is now complemented by the boronium ions 1c and 1e -1g., as shown by voltammetry and the uv spectra of the corresponding radicals.
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A non-radioactive in situ hybridization method is described for the localization of transcription units of defined genes to lateral loops of Xenopus laevis lampbrush chromosomes. Two Xenopus cONA probes were used encoding the nucleolar protein N038/ B23 and cytokeratin 1(8). Both proteins are known to be synthesized in Xenopus oocytes, and Northern blot analysis revealed the presence of the corresponding mRNAs in different oogenic stages. The probes were enzymatically labeled with biotin-dCTP and hybridized to lampbrush chromosomes. The sites of hybridization were detected either by indirect immunofluorescence microscopy using rabbit antibodies against biotin and fluorescein-conjugated antirabbit IgG or enzymatically using peroxidase-conjugated streptavi din. The probe encoding the nucleolar protein hybridized to two sets of lateral loops on different bivalents, the cytokeratin probe to at least four. Our finding that each probe hybridized to more than one chromosomal locus may reflect the tetraploid nature of the Xenopus laevis genome or results from cross-hybridization to other transcriptionally active members of the N038/ B23-nucleoplasmin or the cytokeratin-Iamin gene families. The method described should facilitate further in situ hybridization studies with appropriate genomic clones in order to map specific DNA sequences to defined loop regions and to come to a better understanding of the relationship between loop organization and gene transcription unit.
Many nuclear proteins are released into the cytoplasm at prometaphase and are transported back into the daughter nuclei at the end of mitosis. To determine the role of this reentry in nuclear remodelling during early interphase, we experimentally manipulated nuclear protein uptake in dividing cells. Recently we and others have shown that signal-dependent, pore complex-mediated uptake of nuclear protein is blocked in living cells on microinjection of the lectin wheat germ agglutinin (WGA), or of antibodies such as PI1 that are directed against WGA-binding pore complex glycoproteins. In the present study, we microinjected mitotic PtKz cells with WGA or antibody PIt and followed nuclear reorganization of the daughter cells by immunofluorescence and electron microscopy. The inhibitory effect on nuclear protein uptake was monitored by co-injection of the karyophilic protein nucleoplasmin. When injected by itself early in mitosis, nucleoplasmin became sequestered into the daughter nuclei as they entered telophase. In contrast, nucleoplasmin was excluded from the daughter nuclei in the presence of WGA or antibody PI1 . Although PtKz cells with blocked nuclear protein uptake completed cytokinesis, their nuclei showed a telophaselike organization characterized by highly condensed chromatin surrounded by a nuclear envelope containing a few pore complexes. These findings suggest that pore complexes become functional as early as telophase, in close coincidence with nuclear envelope reformation. They further indicate that the extensive structural rearrangement of the nucleus during the telophase-G1 transition is dependent on the influx of karyophilic proteins from the cytoplasm through the pore complexes, and is not due solely to chromosome- associated components.
Ciliary neurotrophic factor (CNTF) influences the levels of choline acetyltransferase (ChAT) and tyrosine hydroxylase (TH) in cultures of dissociated sYmpathetic neurons from newborn rats. In the presence of CNTF both the total and specific activity of ChAT was increased 7 d after culture by 15- and 18-fold, respectively, as compared to cultures kept in the absence of CNTF. Between 3 and 21 d in culture in the presence of CNTF . the total ChAT activity increased by a factor of >100. Immunotitration demonstrated that the elevated ChAT levels were due to an increased number of enzyme molecules. In contrast to the increase in ChAT levels, the total and specific activity levels' of TH were decreased by 42 and 36 %, respectively, after 7 d in culture. Half-maximal effects for both ChAT increase and TH decrease were obtained at CNTF concentrations of rvO.6 ng and maximal levels were reached at I ng of CNTF per milliliter of medium. The effect of CNTF on TH and ChAT levels were seen in serum-containing medium as well as in serum-free medium. CNTF was shown to have only a small effect on the long-term s.urviVal of rat sympathetic neurons. We therefore concluded that the effects of CNTF on ChAT and TH are not due to selective survival of cells that acquire cholinergic traits in vitro, but are rather due to the induction of cholinergic differentiation of noradrenergic sympathetic neurons.
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Vorwort (Das Kind (1989) 5)
(1989)
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Vorwort (Das Kind (1989) 6)
(1989)
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Jahresbericht 1988
(1989)
Comprehensive geochemical investigations of rnetabasites yielded constraints for a correlation of, or discrimination between the different tectonic units within the northeast Bavarian crystalline basement. The Münchberg nappe pile consists of at least five large tectonic units which exhibit differences in lithology, in part also in metamorphie grade and in metamorphie history. The metabasites in each of these nappes show their own, significant geochemical characteristics. The lowermost tectonic unit, the Bavarian lithofacies, includes the anchimetamorphie Ordovician Randschieferserie which contains alkaline basalts. In their geochemistry, they are sirnilar to the metabasites of the Fichtelgebirge crystalline complex in the autochthonous Saxo-thuringian. The next higher tectonic unit of the Münchberg nappe pile, the Prasinit-Phyllit-Serie contains metabasites which can be derived from subalkaline basalts with a clear calc-alkaline tendency. There is a striking geochemical resemblance to the metabasites of the Erbendorf Greenschist Zone (EGZ) underscorinq the similar lithology of both allochthonous units which appear to be in a similar tectonic position. The Randamphibolit-Serie higher up in the Münchberg nappe pile consists of metabasites with tholeiitic characteristics and a pronounced differentiation trend. The next higher tectonic unit, the Liegendserie of the Münchberg gneiss cornplex s. str., contains metagabbros to metagabbronorites with a high-Al basaltic composition. The amphibolites and banded hornblende gneisses of the overlying Hangendserie are of subalkaline basaltic character with calc-alkaline affinity. The Zone Erbendorf-Vohenstrauss (ZEV) is currently regarded as an allochthonous unit equivalent to the higher crystalline nappes of the Münchberg pile. However, the geochemical character of the metabasites do not encourage such a correlation. Neither the schistose and striped amphibolites nor the flaseramphibolites of the ZEV with their N-KORB and E-MORB character respectively, find convincing counterparts in the crystalline nappes of the Münchberg pile. However, an interestingly close resemblance exists between the schistose and striped amphibolites in the ZEV, on the one hand, and in the autochthonous Zone Tirschenreuth- Mähring (ZTM) and the adjacent Moldanubian sensu strictu, on the other. Owing to the absence of age criteria, our results cannot be used, so far, to reconstruct the paleogeographical position of the individual tectonic units, based on the geochemical characteristics of their respective metabasites.
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Aus einer SE von Guttenberg im Frankenwald auftretenden Abfolge von bisher zur ordovizischen Randschieferserie gezählten grauen Schiefern und Tuffiten, den "Streichengrund-Schichten", wird eine reiche Assoziation organischer Mikrofossilien mit einem unterdevonisehen (Unter-Ems) Ablagerungsalter und mit aufgearbeitetem Unterordovizium (Ober-Arenig) beschrieben. Diese umfaßt 11 Arten der Chlorophyten, 44 Arten der Acritarchen - davon neu Uncillisphaera spinifurcillata 11. sp., sowie 42 Taxa der Sporae dispersae und Scolecodonten. Die somit als tektonische Schuppe innerhalb der ordovizischen Randschieferserie liegenden Streichengrund-Schichten stellen eine im Frankenwald bisher unbekannte Fazieseinheit des Unterdevon dar, die mit ihren vulkanischen Anteilen bayerische, mit ihren klastischen Anteilen thüringische Züge besitzt. Lithologische Vergleiche mit den in streichender Verlängerung nach S hin auftretenden dunklen Schiefern der stratiformen Lagerstätte von Kupferberg legen ein Unterdevon-Alter auch für diese Einheit nahe.
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Observations on captive reed frogs Hyperolius viridijlavus ommatostictus showed that seven out of 24 females changed into males. Sex change occurred without any hormone treatment and resulted in completely functional males. The adaptive value is discussed in terms of maximizing life-time reproductive success. Hyperolius r. ommatostictus is the first amphibian known to show functional sex reversal.
At early developmental stages (embryonic day 7, E7), chick paravertebral sympathetic ganglia contain a cell population that divides in culture while expressing various neuronal properties. In an attempt to identify factors that control neuronal proliferation, we found that ciliary neurotrophic factor (CNTF) specifically inhibits the proliferation of those cells expressing neuronal markers. In addition, CNTF affects the differentiation of sympathetic ganglion cells by inducing the expression of vasoactive intestinal peptide immunoreactivity (VIP-IR). After 1 day in culture, tyrosine hydroxylase immunoreactivity (TH-I R) was expressed by about 86% of the cells whereas VIP-IR was virtually absent. In the presence of CNTF, 50%-60% of the cells expressed VIP-IR after 4 days in culture; however, none of the cells expressed VIP-IR in the absence of CNTF. These results, and the demonstration of cells that express both VIP and TH-IR, indicate that VIP is induced in cells that initially express tyrosine hydroxylase. The findings suggest a potential role for CNTF as a factor affecting the proliferation and differentiation of developing sympathetic neurons.
CILIARY neurotrophic factor (CNTF) was originally characterized as a survival factor for chick ciliary neurons in vitro. More recently, it was shown to promote the survival of a variety of otherneuronal cell types and to affect the differentiation of E7 chick sympathetic neurons by inhibiting their proliferation and by inducing the expression of yasoactiYe intestinal peptide immunoreactiyity (VIP-IR). In cultures of dissociated sympathetic neurons from newborn rats, CNTF induces cholinergic differentiation as shown by increased levels of choline acetyltransferase (ChAT.
Chromosome translocations involving llpl3 have been associated with familial aniridia in two kindreds highlighting the chromosomal localization of the AN2 locus. This locus is also part of the WAGR complex (Wilros tumor, aniridia, genitourinary abnormalities, and mental retardation). In one kindred, the translocation is associated with a deletion, and probes for this region were used to identify and clone the breakpoints of the translocation in the second kindred. Comparison of phage restriction maps exclude the presence of any sizable deletion in this case. Sequences at the chromosome 11 breakpoint are conserved in multiple species, suggesting that the translocation falls within the AN2 gene.
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Die Fries-Chronik des Fürstbischofs Julius Echter von Mespelbrunn : eine fränkische Prachthandschrift des 16. Jahrhunderts aus dem Bestand der Universitätsbibliothek Würzburg Codex M.ch. f.760 ; [Ausstellung zur 500. Wiederkehr des Geburtstages von Magister Lorenz Fries (1489 - 1550), 19. Oktober - 3. Dezember 1989, Universitätsbibliothek Würzburg]
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Ten monoclonal antibodies (McAbs) raised to Schistosoma japonicum eggs could be assigned using several serological and immunochemical techniques to 3 groups. The McAbs, termed A, B and C-McAbs, apparently recognize carbohydrate epitopes that can be located on the same antigen molecule. The antibodies, generally of IgM isotype, are idiotypically related. They are distinct from another IgM McAb (Group D-McAb) the carbohydrate target epitope of which can also be associated with the epitopes of A. B and C-McAbs. The McAbs produce large vacuolated bleb reactions in the circumoval precipitin test (COPT) and target epitopes have different representations in various life cycle stages such as immature and mature eggs, male and female worms (including S. mansoni). Antigens affinity purified on columns containing A, B, C and D-McAbs stimulate proliferation of T cells from egg-sensitized mice and elicit DTH reactions in such mice. This raises the possibility that the target antigens of these carbohydrate-reactive monoclonal antibodies are immunopathologic and involved in egg-induced granuloma formation.
Leishmanien besitzen eine Vielzahl von Mechanismen, die humorale und zelluläre Immunabwehr effektiv zu unterlaufen. Diese hängen eng mit der Expression von hauptsächlich zwei Glykokonjugaten auf der Parasitenoberfläche zusammen, dem gp63 und dem Lipophosphoglykan. Die Parasiten sind einerseits schlechte Aktivatoren des alternativen Komplementweges und umgehen damit ihre eigene extrazelluläre Lyse. Oberflächengebundene Komplementfaktoren fördern andererseits die Aufnahme der Leishmanien durch Makrophagen. Solange diese nicht durch T-Zellen aktiviert sind, dienen sie den Parasiten als "Refugium". Dies gilt insbesondere, als Leishmanien in der Lage sind, 1. den "oxidative burst" zu hemmen; 2. toxische Sauerstoffmetaboliten zu entgiften; 3. abbauende lysosomale Enzyme zu hemmen und 4. das saure Milieu in den Lysosomen für ihren eigenen Metabolismus auszunutzen. Schließlich unterlaufen Leishmanien die zelluläre Immunabwehr des Wirts, indem sie die Aktivierung von T-Lymphozyten hemmen und die Expansion von T-Zell-Sub-populationen bewirken, die für ihr eigenes Überleben nützlich sind.
Although evidence obtained with the PC12 cell line has suggested a role for the ras oncogene proteins in the signal transduction of nerve growth factor-mediated fiber outgrowth, little is known about the signal transduction mechanisms involved in the neuronal response to neurotrophic factors in nontransformed cells. We report here that the oncogene protein T24-ras, when introduced into the cytoplasm of freshly dissociated chick embryonic neurons, promotes the in vitro survival and neurite outgrowth of nerve growth factor-responsive dorsal rootganglion neurons, brain-derived neurotrophic factor-responsive nodose ganglion neurons, and ciliary neuronotrophic factor-responsive ciliary ganglion neurons. The proto-oncogene product c-Ha-ras also promotes neuronal survival, albeit less strongly. No effect could be observed with truncated counterparts of T24-ras and c-Ha-ras lacking the 23 C-terminal amino acids including the membrane-an-choring, palmityl-accepting cysteine. These results sug-gest a generalized involvement of ras or ras-like proteins in the intracellular signal transduction pathway for neurotrophic factors.
Non-Formal Education
(1989)