Refine
Is part of the Bibliography
- yes (19279) (remove)
Year of publication
- 2024 (315)
- 2023 (1036)
- 2022 (1417)
- 2021 (1553)
- 2020 (1228)
- 2019 (987)
- 2018 (919)
- 2017 (732)
- 2016 (862)
- 2015 (875)
- 2014 (901)
- 2013 (806)
- 2012 (828)
- 2011 (732)
- 2010 (573)
- 2009 (436)
- 2008 (428)
- 2007 (407)
- 2006 (418)
- 2005 (398)
- 2004 (391)
- 2003 (307)
- 2002 (276)
- 2001 (163)
- 2000 (82)
- 1999 (21)
- 1998 (6)
- 1997 (4)
- 1996 (7)
- 1995 (12)
- 1994 (215)
- 1993 (214)
- 1992 (197)
- 1991 (177)
- 1990 (159)
- 1989 (147)
- 1988 (132)
- 1987 (122)
- 1986 (106)
- 1985 (74)
- 1984 (69)
- 1983 (58)
- 1982 (53)
- 1981 (50)
- 1980 (57)
- 1979 (48)
- 1978 (41)
- 1977 (36)
- 1976 (40)
- 1975 (28)
- 1974 (19)
- 1973 (26)
- 1972 (24)
- 1971 (14)
- 1970 (14)
- 1969 (10)
- 1968 (8)
- 1967 (2)
- 1965 (3)
- 1964 (2)
- 1963 (2)
- 1961 (1)
- 1845 (1)
Document Type
- Doctoral Thesis (8892)
- Journal article (8296)
- Complete part of issue (703)
- Book article / Book chapter (334)
- Book (197)
- Conference Proceeding (197)
- Preprint (130)
- Review (110)
- Master Thesis (103)
- Report (91)
Language
- English (10793)
- German (8401)
- French (50)
- Multiple languages (21)
- Russian (6)
- Spanish (5)
- Portuguese (2)
- Italian (1)
Keywords
- Würzburg (738)
- Universität (668)
- Wuerzburg (668)
- Wurzburg (660)
- University (608)
- Organische Chemie (135)
- Psychologie (127)
- Anorganische Chemie (124)
- Maus (124)
- Toxikologie (123)
Institute
- Theodor-Boveri-Institut für Biowissenschaften (2266)
- Graduate School of Life Sciences (1014)
- Physikalisches Institut (792)
- Institut für Anorganische Chemie (674)
- Medizinische Klinik und Poliklinik I (647)
- Institut für Psychologie (595)
- Medizinische Klinik und Poliklinik II (556)
- Institut für Organische Chemie (555)
- Neurologische Klinik und Poliklinik (509)
- Klinik und Poliklinik für Allgemein-, Viszeral-, Gefäß- und Kinderchirurgie (Chirurgische Klinik I) (503)
Schriftenreihe
- Cultural Animal Studies, Band 3 (2)
- Spezielle Didaktik der Sportarten (2)
- Alter Orient und Altes Testament : Sonderreihe Veröffentlichungen zur Kultur und Geschichte des Alten Orients ; 3 (1)
- Berichte aus der Informatik (1)
- Deuterocanonical and Cognate Literature Studies (1)
- Deuterocanonical and Cognate Literature Yearbook (1)
- European Journal of Clinical Nutrition ; 70 (1)
- Forum Junge Romanistik 18 (1)
- Grundzüge ; 45 (1)
- International Archives of the History of Ideas / Archives internationales d’histoire des idées 242 (1)
Sonstige beteiligte Institutionen
- Johns Hopkins School of Medicine (18)
- Fraunhofer-Institut für Silicatforschung ISC (8)
- Helmholtz Institute for RNA-based Infection Research (HIRI) (7)
- IZKF Nachwuchsgruppe Geweberegeneration für muskuloskelettale Erkrankungen (7)
- Akademie der Wissenschaften und der Literatur, Mainz (6)
- DFG Forschungsgruppe 2757 / Lokale Selbstregelungen im Kontext schwacher Staatlichkeit in Antike und Moderne (LoSAM) (6)
- Clinical Trial Center (CTC) / Zentrale für Klinische Studien Würzburg (ZKSW) (5)
- Johns Hopkins University School of Medicine (5)
- Universität Leipzig (5)
- Universitätsklinikum Würzburg (5)
ResearcherID
- B-1911-2015 (1)
- B-4606-2017 (1)
- C-2593-2016 (1)
- D-1221-2009 (1)
- D-1250-2010 (1)
- D-3057-2014 (1)
- I-5818-2014 (1)
- J-8841-2015 (1)
- M-1240-2017 (1)
- N-2030-2015 (1)
No abstract available
No abstract available
No abstract available
The plasmid pBC16 (4.25 kbases), ongtnally isolated from Bacillus cereus, determines tetracycline resistance and can be transformed into competent cells of B. subtilis. A miniplasmid of pBCl6 (pBCI6-1), 2,7 kb) which has lost an EcoRI fragment of pBCI6 retains the replication functions and the tetracycline resistance. This plasmid which carries only one EcoRI site has been joined in vitro to pBS], a cryptic plasmid previously isolated from B. subtilis and shown to carry also a single EcoRI site (Bernhard et aI., 1978). The recombinant plasmid is unstable and dissociates into the plasmid pBSl61 (8.2 kb) and the smaller plasmid pBS162 (2. I kb). Plasmid pBS161 retains the tetracycline resistance. It possesses a single EcoRI site and 6 HindlII sites. The largest HindIII fragment of pBS161 carries the tetracycline resistance gene and the replication function. After circularization in vitro of this fragment a new plasmid, pBS161-l is generated, which can be used as a HindlII and EcoRI cloning vector in Bacillus suhtilis. Hybrid plasmids consisting of the E. coli plasmids pBR322, p WL 7 or pACl84 and different HindlII fragments of pBSI61 were constructed in vitro. Hybrids containing together with the E. coli plasmid the largest HindlII fragment of pBS161 can replicate in E. coli and B. sublilis. In E. coli only the replicon of the E. coli plasmid part is functioning whereas in B. suhtilis replication of the hybrid plasmid is under the control of the Bacillus replicon. The tetracycline resistance of the B. subtilis plasmid is expressed in E. coli, but several antibiotic resistances of the E. coli plasmids (ampicillin, kanamycin and chloramphenicol) are not expressed in B. suhtilis. The hybrid plasmids seem to be more unstable in B. subtilis than in E. coli.
The lnfluence of mlcrosomal and nuclear aryl hydrocarbon hydroxylase (AHH) actlvlty on the covalent blndlng of [G·3H]benzo(a )pyrene to rat llver DNA was evaluated in viWJ. lnductlon of mlcrosomal AHH was obtalned alter phenobarbltal treatment (160% of control), whlch also lncreased DNA blndlng to 190%, but left the nuclear actlvlty unchanged. Nuclear AHH was lnduced wlth dleldrln (150%), and the blndlng was decreased to 75%, whereaa the mlcrosomal AHH was at control Ievei. The lncreaslng effect of mlcrosomal AHH lnductlon as weil as the decreaslng effect of nuclear AHH lnductlon on the blndlng was shown clearly when the data of the Individual rata were uaed to solve the equatlon Binding = e•(mlcroeomal AHH) + b•(nuclear AHH) + c Multiple linear regresslon analysls wlth the data from 10 anlmala reaulted ln positive valuea for a and c, a negative value for b, and a good multiple correlatlon coefflclent of r = 0.974. Pretreatment wlth 3-methylcholanthrene ln· duced mlcrosomal AHH to 380% of control and nuclear AHH to 590% and lncreased the blndlng' to 175,.-o. The blndlng was hlgher than predlcted by the formula found, probably because the lncreaslng lnfluence of lnduced mlcrosomal AHH overahadowed the decreaslng effect of the nuclear AHH. The study ahows clearly that the blndlng of a forelgn compound to DNA in viWJ Ia dependent not only on mlcrosomal enzyme actlvltles but also on nuclear actlvltles even lf the latter are conslderably lower than thoae of mlcrosomes.
Thecovalent bindingof [6,7-\(^3\)H]ethinylestradiol (EE)and [6,7-\(^3\)H]estrone (E) to liver DNA of 200 g female ratswas measured 8 h after the administration of 80 \(\mu\)g (9.2 mCi) estrogen by gavage. The binding is 1.5 for EE and 1.1 for E, expressedas binding to DNA/dose, in units of \(\mu\)mol hormonefmol DNA phosphate/mmole honnone/kg body wt. It is in the same order of magnitude as for benzene and about 10 000 tim es below the binding of typical liver carcinogens, such as aflatoxin B\(_1\) or N,N-dimethylnitrosamine.
Wlth radioactive compound of high specific activity, the binding of carcinogene to DNA can be measured wlth doses that are ineffective ln long-term studies. The binding of tritiated benzo(a )pyrene to liver DNA of adult male rats has been determined 50 hr after a singie l.p. injection of doses between 40 1'9/kg and 4 mg/kg. The doseresponse relationship is linear up to 1 mg/kg, shows a step towards 2 mg/kg, and gives a shallow linear slope above that value. The observed binding ranges from 1.7 to 180 nmoles benzo(a)pyrene per mole DNA phosphate. The nonlinearity could be due to an induction of metabolizing enzymes. The microsomal aryl hydrocarbon hydroxylase activity increases significantly 24 hr after a single dose of 4 mg/kg and 48 hr after doses of 2 and 4 mg/kg, but no induction Ia found with 1 mg/kg. The binding from an equimolar dose is 35 times lower than the one found on mouse skin DNA and 300 times lower than that of N,Ndlmethylnitrosamine in rat liver. A good correlatlon exiats to the respective tumor formation in long-term studles.