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No abstract available
Dendritic cells, first described by STEINMAN and COHN in the mouse spleen and now called lymphoid dendritic cells (LDC), were investigated in the rat pancreas with the monoclonal antibodies 29AI-L. T. and MRC-OX17, which both recognize the la-antigen immunohistochemically and immune electron microscopically. la-positive cells with a dendritic morphology were found in the connective tissue of the cxocrine and endocrine pancreas. Immune e1ectron microscopically, the Ia-antibodies were 10- calized on the cell surface and in sm all vesicles. A small portion of the la-positive cells showed additional acid phosphatase positivity, i. e. were la-positive macrophages. The other la-positive cells were probably LDC, which may be important in the elimination of foreign antigens, e. g. bacteria and vIruses.
The \(\alpha\)-Sialyl-\(\beta\) 2-3-Galactosyl-specific adhesin (S adhesin) was isolated from cells of a recombinant Escherichia coli K-12 strain expressing the S-flmbrial adhesin complex. A crude cell extract was partiaUy dissociated into fimbriae and an adhesin-enriched fraction by heating to 7O°C. From the latter, adhesin was purified to apparent homogeneity (by fast protein liquid chromatography, immunoblot, and NaDodSO\(_4\)/PAGE) by differential ammonium sulfate precipitation, dissociation in 8 M guanidine hydrochloride, and high-resolution anion-exchange chromatography in 8 M urea. The purified adhesin formed an aggregate of M\(_r\)\(\approx\)10\(^6\) that was made up of one type of 12-kDa polypeptide (fimbrillin is 16.5 kDa). It had pI value of 4.7 (fimbriae has a pI value of 6). Adhesin and fimbrillin had different amino add compositions. The purified adhesins agglutinated human and bovine erythrocytes with the same speclfkity as the whole bacteria; purified fimbriae were not adhesive. Monoclonal anti-adhesin and anti-fimbriae antibodies were obtained. Monoclonal antiadhesin, but none of the anti-fimbriae, antibodies inhibited the agglutination of erythrocytes. The anti-adhesive antibodies were used in immuno-gold electron microscopy to localize adhesin exclusively on the fimbriae, with a possible preference to their tips.
Pour faire la lumiere as lais? Mittelalterliche Handbücher des Glaubenswissens und ihr Publikum.
(1987)
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