Refine
Has Fulltext
- yes (61)
Is part of the Bibliography
- yes (61)
Year of publication
- 2015 (61) (remove)
Document Type
- Journal article (61) (remove)
Language
- English (61)
Keywords
- membrane proteins (4)
- biodiversity (3)
- cancer (3)
- gene (3)
- in vitro (3)
- mushroom body (3)
- olfaction (3)
- Candida albicans (2)
- ELISPOT (2)
- RNA-Seq (2)
- active zone (2)
- avoidance learning (2)
- bees (2)
- bumblebees (2)
- butterflies (2)
- cytoskeleton (2)
- evolution (2)
- expression (2)
- fluorescent probes (2)
- growth (2)
- honeybee (2)
- insects (2)
- leaf-cutting ants (2)
- localization microscopy (2)
- metabolism (2)
- microglomeruli (2)
- neurotransmitter release (2)
- next generation sequencing (2)
- resistance (2)
- synaptic plasticity (2)
- virulence (2)
- "-omics" (1)
- 3-dimensional structure (1)
- ANOVA (1)
- Acromyrmex ambiguus (1)
- Acyrthosiphon pisum (1)
- Aspergillus fumigatus (1)
- Australian stingless bees (1)
- Axonal degeneration (1)
- BH3 domains (1)
- Bcl-2 proteins (1)
- BioID (1)
- Bruchpilot (1)
- C. elegans (1)
- CD4 (1)
- CD4+T cells (1)
- CD8 (1)
- CD8+T cells (1)
- Ca\(^{2+}\) channels (1)
- Caenorhabditis elegans (1)
- Chlamydia (1)
- Chlamydia trachomatis (1)
- Co-option (1)
- Cynoglossus semilaevis (1)
- DMRT1 (1)
- DNA barcoding (1)
- DNA damage (1)
- DNA methylation (1)
- DNA-binding proteins (1)
- Diabetic polyneuropathy (1)
- Drosophila (1)
- Drosophila melanogaster (1)
- HHblits (1)
- Halobacterium halobium (1)
- HeLa cells (1)
- Homoptera aphididae (1)
- I-tasser (1)
- IFN-γ (1)
- III adenylyl cyclases (1)
- III secretion (1)
- III secretion system (1)
- IL-17 (1)
- IL-2 (1)
- IL-4 (1)
- IL-5 (1)
- ITS2 (1)
- Johnstons organ (1)
- LC-MS/MS (1)
- LINC complex (1)
- Legionella (1)
- MITF (1)
- MORN-repeat (1)
- MYCN (1)
- Motor nerve biopsy (1)
- Myc Transcription (1)
- Mycobacterium (1)
- Mycobacterium tuberculosis (1)
- NF-κB (1)
- NGS (1)
- Neuropathy (1)
- Neurotrophic factors (1)
- Normal Distribution (1)
- SOX9 (1)
- SUN domain proteins (1)
- Salmonella (1)
- Salmonella-containing vacuole (SCV) (1)
- Simkania (1)
- Simkania negevensis (1)
- South Korea (1)
- Squalius alburnoides (1)
- T cells (1)
- T-cells (1)
- TbMORN1 (1)
- Trypanosoma brucei (1)
- V-antigen (1)
- WH2 domain (1)
- Wilms tumour (1)
- Xiphophorus (1)
- Y chromosome (1)
- Yersinia enterocolitica (1)
- \(\alpha\)-latrotoxin (1)
- abundance (1)
- acromyrmex ambiguus (1)
- actin nucleation (1)
- action potentials (1)
- acts downstream (1)
- adaptive growth (1)
- agent-based model (1)
- agricultural intensification (1)
- agricultural landscapes (1)
- agroecosystems (1)
- algorithm (1)
- alignment (1)
- alpha-toxin (1)
- alzheimers disease (1)
- angiogenesis (1)
- animal migration (1)
- animal sexual behavior (1)
- antagonist (1)
- antennal lobe (1)
- antimicrobial peptides (1)
- apis mellifera (1)
- apixaban (1)
- apoptosis (1)
- armyworm Lepidoptera (1)
- assemblages (1)
- bacterial invasion (1)
- bacterial pathogens (1)
- bacteriorhodopsin (1)
- bacterium Legionella pneumophila (1)
- bagworms Lepidoptera (1)
- balance hypothesis (1)
- bark beetles (1)
- bax (1)
- beetle horns (1)
- behavior (1)
- behavioral conditioning (1)
- beta-catenin (1)
- beta-diveristy (1)
- beta-lactamase inhibition (1)
- binding (1)
- biodiversity conservation (1)
- biodiversity-ecosystem functioning (1)
- biofilm formation (1)
- bioinformatics and computational biology (1)
- biological control (1)
- biology (1)
- biomaterial surfaces (1)
- body-size (1)
- cabbage Brassica oleracea var. capitata (1)
- camponotus floridanus (1)
- candida genome database (1)
- carbon dioxide avoidance (1)
- carbonaria (1)
- carpenter ant (1)
- carrying capacity (1)
- cell fate (1)
- cell membranes (1)
- cell proliferation (1)
- cell size (1)
- cell wall (1)
- cells (1)
- cellular camp (1)
- cellular senescence (1)
- centrality (1)
- channelrhodopsin-2 (1)
- chlamydia (1)
- chlamydia infection (1)
- chlamydia trachomatis (1)
- chondrosarcoma (1)
- chordotonal organs (1)
- circadian rhythms (1)
- climate change (1)
- clock reproduction ecology (1)
- co-immunoprecipitation (1)
- coincidence (1)
- color discrimination (1)
- communities (1)
- community functional-responses (1)
- compartment boundary (1)
- complex (1)
- complex networks (1)
- complex traits (1)
- compound eye (1)
- computational prediction (1)
- computational systems biology (1)
- contributes (1)
- control profiles (1)
- copy number (1)
- crystal structure (1)
- cuticular profiles (1)
- cytokine profiling (1)
- cytokines (1)
- cytokinesis (1)
- c‐Myc (1)
- dSTORM (1)
- database (1)
- day length (1)
- death pathway sar locus (1)
- decay (1)
- decline (1)
- defense and evasion strategies (1)
- defenses (1)
- degradation (1)
- dendritic cells (1)
- determination locus (1)
- determining genes (1)
- diagnostic accuracy (1)
- differentially expressed genes (1)
- differentiation (1)
- diffraction limit (1)
- direct oral anticoagulants (1)
- direct thrombin inhibitor (1)
- discovery (1)
- disruption project (1)
- diversity (1)
- domain (1)
- drosophila larvae (1)
- dynamics (1)
- ecology (1)
- ecosystem services (1)
- electrolytes (1)
- encephalitis dementia (1)
- endocytic multivesicular bodies (1)
- endoplasmic reticulum (1)
- endothelial cells (1)
- enteric pathogens (1)
- enterica serovar Typhimurium (1)
- envelopment (1)
- epithelial cells (1)
- eucalyptus (1)
- european beech forests (1)
- european countries (1)
- excitation-secretion coupling (1)
- exome (1)
- expression analysis (1)
- factor B (1)
- factor XA inhibitor (1)
- family (1)
- fish (1)
- fishes Xiphophorus (1)
- flagellar motility (1)
- flies (1)
- flow (1)
- flowers (1)
- fluorescence microscopy (1)
- format (1)
- fruit set (1)
- functional analysis (1)
- functional modules (1)
- fungal pathogens (1)
- gain (1)
- gating (1)
- gene expression (1)
- gene ontology (1)
- gene regulator agr (1)
- genetics (1)
- genome (1)
- genome browser (1)
- genome cells (1)
- genus Xiphophorus (1)
- glial cells (1)
- glycerol (1)
- gonopodium (1)
- growth and differentiation factor 5 (1)
- guides (1)
- habitats (1)
- hearing (1)
- herbivores (1)
- herbivorous insects (1)
- heterogamety (1)
- heterogenity (1)
- heterosis (1)
- high throughput sequencing (1)
- histology (1)
- honey bees (1)
- host cells (1)
- host pathogen interactions (1)
- host-pathogen adaption (1)
- host-pathogen interaction (1)
- human african trypanosomiasis (1)
- human immune system (1)
- human immunodeficiency virus (1)
- human melanoma (1)
- hybrid origin (1)
- hybridization (1)
- hybrids (1)
- hymenoptera (1)
- hypotonic (1)
- hypotonic solutions (1)
- hypoxia-independent (1)
- illumina MiSeq platform (1)
- illumination microscopy (1)
- imaginal disk (1)
- immune system (1)
- immuno-magnetic purification (1)
- immunological cross-talk (1)
- immunoprecipitation (1)
- import (1)
- in vivo (1)
- infection (1)
- insect (1)
- insect populations (1)
- insect timing (1)
- instinct (1)
- integrative genomics viewer (1)
- interolog (1)
- intracellular bacteria (1)
- intracellular pH (1)
- isotonic (1)
- isotopolog profiling (1)
- kappa-B (1)
- key innovation (1)
- land use intensification (1)
- land-use intensity (1)
- learning (1)
- life span (1)
- ligand CD55 (1)
- ligand-receptor complex (1)
- linkage map (1)
- long-distance dispersal (1)
- maize (1)
- male-specific traits (1)
- males emerge (1)
- managed grasslands (1)
- management (1)
- mating success (1)
- melanogenesis (1)
- melanoma (1)
- membrane potential (1)
- membrane receptor signaling (1)
- memory (1)
- memory immune responses (1)
- metaanalysis (1)
- metastasis (1)
- metastatic melanoma (1)
- mice (1)
- microarray (1)
- microbial rhodopsins (1)
- mitochondria (1)
- mitochondrial membrane (1)
- models (1)
- modulates virulence (1)
- molecular cloning (1)
- monoclonal antibodies (1)
- morphogenetic furrow progression (1)
- mortality rates (1)
- moths and butterflies (1)
- motor proteins (1)
- mouse model (1)
- mouse testis differentiation (1)
- multi-electrode-recording (1)
- multiparticle collision dynamics (1)
- mutants (1)
- mutation (1)
- native bees (1)
- natural variation (1)
- nectar (1)
- network inference (1)
- networks (1)
- neuromuscular junction (1)
- neurons (1)
- nitric oxide (1)
- nonhuman-primates (1)
- normal distribution (1)
- norway spruce (1)
- nuclear envelope (1)
- nuclear pore complex (1)
- nucleotide-gated channel (1)
- odorants (1)
- oncogene amplification (1)
- oncogene-induced senescence (1)
- optical control (1)
- optical reconstruction microscopy (1)
- organismal evolution (1)
- osmia (1)
- outer membrane proteins (1)
- painful (1)
- palynolog (1)
- parasites (1)
- pathogen vacuole (1)
- pathogen-host interaction (PHI) (1)
- pathogenicity (1)
- pathways (1)
- performance liquid chromatography (1)
- permeability (1)
- pest (1)
- pestis infection (1)
- phagosome maturation arrest (1)
- phenology shifts (1)
- phenotypic plasticity (1)
- pheromones (1)
- phosphorylation (1)
- photodynamic therapy (1)
- physiological constraints (1)
- plant diversity (1)
- plant-insect interactions (1)
- plantations (1)
- pneumonic plague (1)
- poeciliid fishes (1)
- pollen (1)
- pollination (1)
- pollination ecology (1)
- populations (1)
- potential role (1)
- preexisting bias (1)
- preproteins (1)
- presynaptic calcium (1)
- presynaptic differentiation (1)
- principal component analysis (1)
- productivity (1)
- prognostic marker (1)
- proportion of seminatural habitat (1)
- propulsion (1)
- protandry (1)
- protein biosynthesis & quality control (1)
- protein complexes (1)
- protein domains (1)
- protein interaction database (1)
- protein interactions (1)
- protein-coupled receptors (1)
- protein-protein interaction (1)
- protein-protein interaction network (1)
- proteomics (1)
- quantification (1)
- rare (1)
- re-annotation (1)
- reactive oxygen (1)
- reactive oxygen species (1)
- recognize images (1)
- recombinant protein rVE (1)
- reconstruction microscopy (1)
- regulation (1)
- regulatory networks (1)
- reporter gene (1)
- resin (1)
- resolution (1)
- retinal differentiation (1)
- reveals (1)
- rhodopsin (1)
- ribosome (1)
- sequence alignment (1)
- sequencing data (1)
- serum (1)
- sex chromosome evolution (1)
- sex combs (1)
- sex differentiation (1)
- shear stress (1)
- shootin-1 (1)
- signals (1)
- similarity (1)
- size dimorphism (1)
- skin (1)
- small-colony variants (1)
- snoRNA (1)
- software (1)
- somatic mutations (1)
- specialization (1)
- species richness (1)
- spectrometry-based proteomics (1)
- sperm head formation (1)
- spermatogenesis (1)
- spermiogenesis (1)
- spire (1)
- spot size (1)
- stage III (1)
- statistical disperison (1)
- statistics (1)
- strategy (1)
- structure-function relationships (1)
- structured illumination (1)
- substrate quality (1)
- sucrose (1)
- super-resolution microscopy (1)
- sustainable intensification (1)
- swordtails (1)
- synaptic delay (1)
- synaptic transmission (1)
- synaptotagmin (1)
- t-Test (1)
- tag fusion proteins (1)
- tegument protein pUL36 (1)
- teleostei (1)
- temperature (1)
- tonicity (1)
- transcription (1)
- transcription factors (1)
- transcriptional regulation (1)
- transcriptional responses (1)
- transcriptome (1)
- transformation (1)
- translational regulation (1)
- trees (1)
- trophic interactions (1)
- tsetse fly (1)
- tumors (1)
- turnover (1)
- type-1 (1)
- ubiquitination (1)
- vaccine (1)
- validation (1)
- variants (1)
- vision (1)
Institute
- Theodor-Boveri-Institut für Biowissenschaften (61) (remove)
Sonstige beteiligte Institutionen
ResearcherID
- D-1221-2009 (1)
Long-term behavioral changes related to learning and experience have been shown to be associated with structural remodeling in the brain. Leaf-cutting ants learn to avoid previously preferred plants after they have proved harmful for their symbiotic fungus, a process that involves long-term olfactory memory. We studied the dynamics of brain microarchitectural changes after long-term olfactory memory formation following avoidance learning in Acromyrmex ambiguus. After performing experiments to control for possible neuronal changes related to age and body size, we quantified synaptic complexes (microglomeruli, MG) in olfactory regions of the mushroom bodies (MB) at different times after learning. Long-term avoidance memory formation was associated with a transient change in MG densities. Two days after learning, MG density was higher than before learning. At days 4 and 15 after learning when ants still showed plant avoidance MG densities had decreased to the initial state. The structural reorganization of MG triggered by long-term avoidance memory formation clearly differed from changes promoted by pure exposure to and collection of novel plants with distinct odors. Sensory exposure by the simultaneous collection of several, instead of one, non-harmful plant species resulted in a decrease in MG densities in the olfactory lip. We hypothesize that while sensory exposure leads to MG pruning in the MB olfactory lip, the formation of long-term avoidance memory involves an initial growth of new MG followed by subsequent pruning.
In vitro evidence for senescent multinucleated melanocytes as a source for tumor-initiating cells
(2015)
Oncogenic signaling in melanocytes results in oncogene-induced senescence (OIS), a stable cell-cycle arrest frequently characterized by a bi-or multinuclear phenotype that is considered as a barrier to cancer progression. However, the long-sustained conviction that senescence is a truly irreversible process has recently been challenged. Still, it is not known whether cells driven into OIS can progress to cancer and thereby pose a potential threat. Here, we show that prolonged expression of the melanoma oncogene N-RAS\(^{61K}\) in pigment cells overcomes OIS by triggering the emergence of tumor-initiating mononucleated stem-like cells from senescent cells. This progeny is dedifferentiated, highly proliferative, anoikis-resistant and induces fast growing, metastatic tumors. Our data describe that differentiated cells, which are driven into senescence by an oncogene, use this senescence state as trigger for tumor transformation, giving rise to highly aggressive tumor-initiating cells. These observations provide the first experimental in vitro evidence for the evasion of OIS on the cellular level and ensuing transformation.
G-protein-coupled receptors (GPCRs) are typically regarded as chemosensors that control cellular states in response to soluble extracellular cues. However, the modality of stimuli recognized through adhesion GPCR (aGPCR), the second largest class of the GPCR superfamily, is unresolved. Our study characterizes the Drosophila aGPCR Latrophilin/dCirl, a prototype member of this enigmatic receptor class. We show that dCirl shapes the perception of tactile, proprioceptive, and auditory stimuli through chordotonal neurons, the principal mechanosensors of Drosophila. dCirl sensitizes these neurons for the detection of mechanical stimulation by amplifying their input-output function. Our results indicate that aGPCR may generally process and modulate the perception of mechanical signals, linking these important stimuli to the sensory canon of the GPCR superfamily.
Control of genetic regulatory networks is challenging to define and quantify. Previous control centrality metrics, which aim to capture the ability of individual nodes to control the system, have been found to suffer from plausibility and applicability problems. Here we present a new approach to control centrality based on network convergence behaviour, implemented as an extension of our genetic regulatory network simulation framework Jimena (http://stefan-karl.de/jimena). We distinguish three types of network control, and show how these mathematical concepts correspond to experimentally verified node functions and signalling pathways in immunity and cell differentiation: Total control centrality quantifies the impact of node mutations and identifies potential pharmacological targets such as genes involved in oncogenesis (e.g. zinc finger protein GLI2 or bone morphogenetic proteins in chondrocytes). Dynamic control centrality describes relaying functions as observed in signalling cascades (e.g. src kinase or Jak/Stat pathways). Value control centrality measures the direct influence of the value of the node on the network (e.g. Indian hedgehog as an essential regulator of proliferation in chondrocytes). Surveying random scale-free networks and biological networks, we find that control of the network resides in few high degree driver nodes and networks can be controlled best if they are sparsely connected.
Aphids are a major concern in agricultural crops worldwide, and control by natural enemies is an essential component of the ecological intensification of agriculture. Although the complexity of agricultural landscapes is known to influence natural enemies of pests, few studies have measured the degree of pest control by different enemy guilds across gradients in landscape complexity. Here, we use multiple natural-enemy exclosures replicated in 18 fields across a gradient in landscape complexity to investigate (1) the strength of natural pest control across landscapes, measured as the difference between pest pressure in the presence and in the absence of natural enemies; (2) the differential contributions of natural enemy guilds to pest control, and the nature of their interactions across landscapes. We show that natural pest control of aphids increased up to six-fold from simple to complex landscapes. In the absence of pest control, aphid population growth was higher in complex than simple landscapes, but was reduced by natural enemies to similar growth rates across all landscapes. The effects of enemy guilds were landscape-dependent. Particularly in complex landscapes, total pest control was supplied by the combined contribution of flying insects and ground-dwellers. Birds had little overall impact on aphid control. Despite evidence for intraguild predation of flying insects by ground-dwellers and birds, the overall effect of enemy guilds on aphid control was complementary. Understanding pest control services at large spatial scales is critical to increase the success of ecological intensification schemes. Our results suggest that, where aphids are the main pest of concern, interactions between natural enemies are largely complementary and lead to a strongly positive effect of landscape complexity on pest control. Increasing the availability of seminatural habitats in agricultural landscapes may thus benefit not only natural enemies, but also the effectiveness of aphid natural pest control.
Climate change can alter the phenology of organisms. It may thus lead seasonal organisms to face different day lengths than in the past, and the fitness consequences of these changes are as yet unclear. To study such effects, we used the pea aphid Acyrthosiphon pisum as a model organism, as it has obligately asexual clones which can be used to study day length effects without eliciting a seasonal response. We recorded life-history traits under short and long days, both with two realistic temperature cycles with means differing by 2 °C. In addition, we measured the population growth of aphids on their host plant Pisum sativum. We show that short days reduce fecundity and the length of the reproductive period of aphids. Nevertheless, this does not translate into differences at the population level because the observed fitness costs only become apparent late in the individual's life. As expected, warm temperature shortens the development time by 0.7 days/°C, leading to faster generation times. We found no interaction of temperature and day length. We conclude that day length changes cause only relatively mild costs, which may not decelerate the increase in pest status due to climate change.
Fungal microorganisms frequently lead to life-threatening infections. Within this group of pathogens, the commensal Candida albicans and the filamentous fungus Aspergillus fumigatus are by far the most important causes of invasive mycoses in Europe. A key capability for host invasion and immune response evasion are specific molecular interactions between the fungal pathogen and its human host. Experimentally validated knowledge about these crucial interactions is rare in literature and even specialized host pathogen databases mainly focus on bacterial and viral interactions whereas information on fungi is still sparse. To establish large-scale host fungi interaction networks on a systems biology scale, we develop an extended inference approach based on protein orthology and data on gene functions. Using human and yeast intraspecies networks as template, we derive a large network of pathogen host interactions (PHI). Rigorous filtering and refinement steps based on cellular localization and pathogenicity information of predicted interactors yield a primary scaffold of fungi human and fungi mouse interaction networks. Specific enrichment of known pathogenicity-relevant genes indicates the biological relevance of the predicted PHI. A detailed inspection of functionally relevant subnetworks reveals novel host fungal interaction candidates such as the Candida virulence factor PLB1 and the anti-fungal host protein APP. Our results demonstrate the applicability of interolog-based prediction methods for host fungi interactions and underline the importance of filtering and refinement steps to attain biologically more relevant interactions. This integrated network framework can serve as a basis for future analyses of high-throughput host fungi transcriptome and proteome data.
Cyclic GMP (cGMP) signalling regulates multiple biological functions through activation of protein kinase G and cyclic nucleotide-gated (CNG) channels. In sensory neurons, cGMP permits signal modulation, amplification and encoding, before depolarization. Here we implement a guanylyl cyclase rhodopsin from Blastocladiella emersonii as a new optogenetic tool (BeCyclOp), enabling rapid light-triggered cGMP increase in heterologous cells (Xenopus oocytes, HEK293T cells) and in Caenorhabditis elegans. Among five different fungal CyclOps, exhibiting unusual eight transmembrane topologies and cytosolic N-termini, BeCyclOp is the superior optogenetic tool (light/dark activity ratio: 5,000; no cAMP production; turnover (20 °C) ~17 cGMPs\(^{-1}\)). Via co-expressed CNG channels (OLF in oocytes, TAX-2/4 in C. elegans muscle), BeCyclOp photoactivation induces a rapid conductance increase and depolarization at very low light intensities. In O\(_2\)/CO\(_2\) sensory neurons of C. elegans, BeCyclOp activation evokes behavioural responses consistent with their normal sensory function. BeCyclOp therefore enables precise and rapid optogenetic manipulation of cGMP levels in cells and animals.
Super-resolution microscopy can unravel previously hidden details of cellular structures but requires high irradiation intensities to use the limited photon budget efficiently. Such high photon densities are likely to induce cellular damage in live-cell experiments. We applied single-molecule localization microscopy conditions and tested the influence of irradiation intensity, illumination-mode, wavelength, light-dose, temperature and fluorescence labeling on the survival probability of different cell lines 20-24 hours after irradiation. In addition, we measured the microtubule growth speed after irradiation. The photo-sensitivity is dramatically increased at lower irradiation wavelength. We observed fixation, plasma membrane permeabilization and cytoskeleton destruction upon irradiation with shorter wavelengths. While cells stand light intensities of similar to 1 kW cm\(^{-2}\) at 640 nm for several minutes, the maximum dose at 405 nm is only similar to 50 J cm\(^{-2}\), emphasizing red fluorophores for live-cell localization microscopy. We also present strategies to minimize phototoxic factors and maximize the cells ability to cope with higher irradiation intensities.
Freshly cut beech deadwood was enriched in the canopy and on the ground in three cultural landscapes in Germany (Swabian Alb, Hainich-Dun, Schorfheide-Chorin) in order to analyse the diversity, distribution and interaction of wood-inhabiting fungi and beetles. After two years of wood decay 83 MOTUs (Molecular Operational Taxonomic Units) from 28 wood samples were identified. Flight Interception Traps (FITs) installed adjacent to the deadwood enrichments captured 29.465 beetles which were sorted to 566 species. Geographical 'region' was the main factor determining both beetle and fungal assemblages. The proportions of species occurring in all regions were low. Statistic models suggest that assemblages of both taxa differed between stratum and management praxis but their strength varied among regions. Fungal assemblages in Hainich-Dun, for which the data was most comprehensive, discriminated unmanaged from extensively managed and age-class forests (even-aged timber management) while canopy communities differed not from those near the ground. In contrast, the beetle assemblages at the same sites showed the opposite pattern. We pursued an approach in the search for fungus-beetle associations by computing cross correlations and visualize significant links in a network graph. These correlations can be used to formulate hypotheses on mutualistic relationships for example in respect to beetles acting as vectors of fungal spores.