Polyoxazolines with a vicinally double-bioactivated terminus for biomacromolecular affinity assessment
Zitieren Sie bitte immer diese URN: urn:nbn:de:bvb:20-opus-239530
- Interactions between proteins and carbohydrates with larger biomacromolecules, e.g., lectins, are usually examined using self-assembled monolayers on target gold surfaces as a simplified model measuring setup. However, most of those measuring setups are either limited to a single substrate or do not allow for control over ligand distance and spacing. Here, we develop a synthetic strategy, consisting of a cascade of a thioesterification, native chemical ligation (NCL) and thiol-ene reaction, in order to create three-component polymer conjugatesInteractions between proteins and carbohydrates with larger biomacromolecules, e.g., lectins, are usually examined using self-assembled monolayers on target gold surfaces as a simplified model measuring setup. However, most of those measuring setups are either limited to a single substrate or do not allow for control over ligand distance and spacing. Here, we develop a synthetic strategy, consisting of a cascade of a thioesterification, native chemical ligation (NCL) and thiol-ene reaction, in order to create three-component polymer conjugates with a defined double bioactivation at the chain end. The target architecture is the vicinal attachment of two biomolecule residues to the α telechelic end point of a polymer and a thioether group at the ω chain end for fixating the conjugate to a gold sensor chip surface. As proof-of-principle studies for affinity measurements, we demonstrate the interaction between covalently bound mannose and ConA in surface acoustic wave (SAW) and surface plasmon resonance (SPR) experiments.…
Autor(en): | Florian Pinzner, Thorsten Keller, Jürgen Mut, Julian Bechold, Jürgen Seibel, Jürgen Groll |
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URN: | urn:nbn:de:bvb:20-opus-239530 |
Dokumentart: | Artikel / Aufsatz in einer Zeitschrift |
Institute der Universität: | Fakultät für Chemie und Pharmazie / Institut für Organische Chemie |
Medizinische Fakultät / Abteilung für Funktionswerkstoffe der Medizin und der Zahnheilkunde | |
Sprache der Veröffentlichung: | Englisch |
Titel des übergeordneten Werkes / der Zeitschrift (Englisch): | Sensors |
ISSN: | 1424-8220 |
Erscheinungsjahr: | 2021 |
Band / Jahrgang: | 21 |
Heft / Ausgabe: | 9 |
Aufsatznummer: | 3153 |
Originalveröffentlichung / Quelle: | Sensors (2021) 21:9, 3153. https://doi.org/10.3390/s21093153 |
DOI: | https://doi.org/10.3390/s21093153 |
Allgemeine fachliche Zuordnung (DDC-Klassifikation): | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
Freie Schlagwort(e): | functionalization; lectin; polyoxazolines |
Datum der Freischaltung: | 13.07.2022 |
Datum der Erstveröffentlichung: | 01.05.2021 |
Lizenz (Deutsch): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |