Do the enantiomers of ketamine bind enantioselectively to human serum albumin?
Please always quote using this URN: urn:nbn:de:bvb:20-opus-349791
- The binding of drugs to plasma proteins is an important process in the human body and has a significant influence on pharmacokinetic parameter. Human serum albumin (HSA) has the most important function as a transporter protein. The binding of ketamine to HSA has already been described in literature, but only of the racemate. The enantiomerically pure S-ketamine is used as injection solution for induction of anesthesia and has been approved by the Food and Drug Administration for the therapy of severe depression as a nasal spray in 2019. TheThe binding of drugs to plasma proteins is an important process in the human body and has a significant influence on pharmacokinetic parameter. Human serum albumin (HSA) has the most important function as a transporter protein. The binding of ketamine to HSA has already been described in literature, but only of the racemate. The enantiomerically pure S-ketamine is used as injection solution for induction of anesthesia and has been approved by the Food and Drug Administration for the therapy of severe depression as a nasal spray in 2019. The question arises if there is enantioselective binding to HSA. Hence, the aim of this study was to investigate whether there is enantioselective binding of S-and R-ketamine to HSA or not. Ultrafiltration (UF) followed by chiral capillary electrophoretic analysis was used to determine the extent of protein binding. Bound fraction to HSA was 71.2 % and 64.9 % for enantiomerically pure R- and S-ketamine, respectively, and 66.5 % for the racemate. Detailed binding properties were studied by Saturation Transfer Difference (STD)-, waterLOGSY- and Carr-Purcell-Meiboom-Gill (CPMG)-NMR spectroscopy. With all three methods, the aromatic ring and the N-methyl group could be identified as the structural moieties most strongly involved in binding of ketamine to HSA. pK\(_{aff}\) values determined using UF and NMR indicate that ketamine is a weak affinity ligand to HSA and no significant differences in binding behavior were found between the individual enantiomers and the racemate.…
Author: | Sebastian Schmidt, Ulrike Holzgrabe |
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URN: | urn:nbn:de:bvb:20-opus-349791 |
Document Type: | Journal article |
Faculties: | Fakultät für Chemie und Pharmazie / Institut für Pharmazie und Lebensmittelchemie |
Language: | English |
Parent Title (English): | European Journal of Pharmaceutical Sciences |
Year of Completion: | 2024 |
Volume: | 192 |
Article Number: | 106640 |
Source: | European Journal of Pharmaceutical Sciences (2024) 192:106640. DOI: 10.1016/j.ejps.2023.106640 |
DOI: | https://doi.org/10.1016/j.ejps.2023.106640 |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
Tag: | albumin; electrophoresis; enantioselectivity; nuclear magnetic resonance spectroscopy; protein binding; ultrafiltration |
Release Date: | 2024/05/15 |
Licence (German): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |