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A First Vascularized Skin Equivalent as an Alternative to Animal Experimentation

Please always quote using this URN: urn:nbn:de:bvb:20-opus-164438
  • Tissue-engineered skin equivalents mimic key aspects of the human skin, and can thus be employed as wound coverage for large skin defects or as in vitro test systems as an alternative to animal models. However, current skin equivalents lack a functional vasculature limiting clinical and research applications. This study demonstrates the generation of a vascularized skin equivalent with a perfused vascular network by combining a biological vascularized scaffold (BioVaSc) based on a decellularized segment of a porcine jejunum and a tailoredTissue-engineered skin equivalents mimic key aspects of the human skin, and can thus be employed as wound coverage for large skin defects or as in vitro test systems as an alternative to animal models. However, current skin equivalents lack a functional vasculature limiting clinical and research applications. This study demonstrates the generation of a vascularized skin equivalent with a perfused vascular network by combining a biological vascularized scaffold (BioVaSc) based on a decellularized segment of a porcine jejunum and a tailored bioreactor system. Briefly, the BioVaSc was seeded with human fibroblasts, keratinocytes, and human microvascular endothelial cells. After 14 days at the air-liquid interface, hematoxylin & eosin and immunohistological staining revealed a specific histological architecture representative of the human dermis and epidermis including a papillary-like architecture at the dermal-epidermal-junction. The formation of the skin barrier was measured non-destructively using impedance spectroscopy. Additionally, endothelial cells lined the walls of the formed vessels that could be perfused with a physiological volume flow. Due to the presence of a complex in-vivo-like vasculature, the here shown skin equivalent has the potential for skin grafting and represents a sophisticated in vitro model for dermatological research.show moreshow less

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Metadaten
Author: Florian Groeber, Lisa Engelhardt, Julia Lange, Szymon Kurdyn, Freia F. Schmid, Christoph Rücker, Stephan Mielke, Heike Walles, Jan Hansmann
URN:urn:nbn:de:bvb:20-opus-164438
Document Type:Journal article
Faculties:Medizinische Fakultät / Medizinische Klinik und Poliklinik II
Medizinische Fakultät / Lehrstuhl für Tissue Engineering und Regenerative Medizin
Language:English
Parent Title (English):ALTEX - Alternatives to Animal Experimentation
Year of Completion:2016
Volume:33
Issue:4
Pagenumber:415-422
Source:ALTEX 2016, 33(4), 415-422
DOI:https://doi.org/10.14573/altex.1604041
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Tag:alternative to animal testing; skin equivalents; tissue engineering; vascularization
Release Date:2019/09/20
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International