Single-Molecule Ultrafast Fluorescence-Detected Pump–Probe Microscopy
Please always quote using this URN: urn:nbn:de:bvb:20-opus-313485
- We introduce fluorescence-detected pump–probe microscopy by combining a wavelength-tunable ultrafast laser with a confocal scanning fluorescence microscope, enabling access to the femtosecond time scale on the micrometer spatial scale. In addition, we obtain spectral information from Fourier transformation over excitation pulse-pair time delays. We demonstrate this new approach on a model system of a terrylene bisimide (TBI) dye embedded in a PMMA matrix and acquire the linear excitation spectrum as well as time-dependent pump–probe spectraWe introduce fluorescence-detected pump–probe microscopy by combining a wavelength-tunable ultrafast laser with a confocal scanning fluorescence microscope, enabling access to the femtosecond time scale on the micrometer spatial scale. In addition, we obtain spectral information from Fourier transformation over excitation pulse-pair time delays. We demonstrate this new approach on a model system of a terrylene bisimide (TBI) dye embedded in a PMMA matrix and acquire the linear excitation spectrum as well as time-dependent pump–probe spectra simultaneously. We then push the technique towards single TBI molecules and analyze the statistical distribution of their excitation spectra. Furthermore, we demonstrate the ultrafast transient evolution of several individual molecules, highlighting their different behavior in contrast to the ensemble due to their individual local environment. By correlating the linear and nonlinear spectra, we assess the effect of the molecular environment on the excited-state energy.…
Author: | Daniel Fersch, Pavel Malý, Jessica Rühe, Victor Lisinetskii, Matthias Hensen, Frank WürthnerORCiDGND, Tobias Brixner |
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URN: | urn:nbn:de:bvb:20-opus-313485 |
Document Type: | Preprint |
Faculties: | Fakultät für Chemie und Pharmazie / Institut für Organische Chemie |
Fakultät für Chemie und Pharmazie / Institut für Physikalische und Theoretische Chemie | |
Language: | English |
Year of Completion: | 2023 |
DOI: | https://doi.org/10.25972/OPUS-31348 |
Sonstige beteiligte Institutionen: | Charles University, Faculty of Mathematics and Physics, Ke Karlovu 5, 121 16 Prague, Czech Republic |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 541 Physikalische Chemie |
GND Keyword: | Fluoreszenz |
Tag: | Single-molecule microscopy; Ultrafast spectroscopy |
Release Date: | 2023/05/10 |
EU-Project number / Contract (GA) number: | 101030656 |
OpenAIRE: | OpenAIRE |
Licence (German): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |